Difference between revisions of "DNA Microarray Analysis (academic course)"
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The technology behind GeneChips and cDNA arrays; normalization of data before comparison between experiments; statistical significance of changes in expression; reduction and visualization of large data matrices: PCA and clustering; reverse engineering of regulatory networks; promoter analysis and functional analysis; integration of data; case stories. Finally a poster presentation based on a problem formulated in cooperation with the teachers. | The technology behind GeneChips and cDNA arrays; normalization of data before comparison between experiments; statistical significance of changes in expression; reduction and visualization of large data matrices: PCA and clustering; reverse engineering of regulatory networks; promoter analysis and functional analysis; integration of data; case stories. Finally a poster presentation based on a problem formulated in cooperation with the teachers. | ||
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+ | === Textbook === | ||
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+ | * '''''[[Guide to Analysis of DNA Microarray Data]]''''', Second Edition (Paperback) — by Steen Knudsen | ||
===Remarks=== | ===Remarks=== |
Revision as of 21:30, 4 January 2006
Contents
- 1 Overview
- 2 Course Programme
- 2.1 2-Jan-2006 (Monday): Introduction to microarray technology and R software
- 2.2 3-Jan-2006 (Tuesday): Preprocessing and Bioconductor
- 2.3 4-Jan-2006 (Wednesday): Data Normalization
- 2.4 5-Jan-2006 (Thursday): Statistical analysis
- 2.5 6-Jan-2006 (Friday): Data visualization and class prediction
- 2.6 9-Jan-2006 (Monday): Gene annotation analysis
- 2.7 10-Jan-2006 (Tuesday): Integrative Biology
- 2.8 11-Jan-2006 (Wednesday): Project Problem Formulation
- 2.9 12-Jan-2006 (Thursday): Problem Presentations
- 2.10 13-Jan-2006 (Friday) — 19-Jan-2006 (Thursday): Project Work
- 2.11 20-Jan-2006 (Friday): Poster Session
- 2.12 18-Jan-2006 (Wednesday) — 21-Jan-2006 (Saturday)
Overview
Aim/objectives
The DNA microarray technology plays a significant role in molecular biology, systems biology and pharmaceutical research. The aim of this course is to give students a general knowledge of methods for microarray analysis as well as the ability to apply computerized analyses on gene expression data. Furthermore, it is a goal that the students learn to formulate interesting problems/questions that can be addressed by gene expressions analysis
Content
The technology behind GeneChips and cDNA arrays; normalization of data before comparison between experiments; statistical significance of changes in expression; reduction and visualization of large data matrices: PCA and clustering; reverse engineering of regulatory networks; promoter analysis and functional analysis; integration of data; case stories. Finally a poster presentation based on a problem formulated in cooperation with the teachers.
Textbook
- Guide to Analysis of DNA Microarray Data, Second Edition (Paperback) — by Steen Knudsen
Remarks
The evaluated exercises/projects will be done in groups and the work done by each student must be identified, so that each student can receive and individual grade.
Contact
- Professor: Henrik Bjørn Nielsen, build. 208, room 018, (+45) 4525 2489, hbjorn@cbs.dtu.dk
- Department: 27 BioCentrum-DTU
- Home Page: http://www.cbs.dtu.dk/chipcourse/
- Course code: 27612
Key words
DNA Microarrays, Expression Analysis, GeneChip, Regulatory networks, classification, systems biology
Course Programme
The course will be held from January 2 - 20, 2006 (incl.)
Lectures and exercises will take place at the Center for Biological Sequence Analysis at the Technical University of Denmark, building 208, auditorium 51, in Lyngby.
- Beginner's Guide to UNIX is available on-line.
- An Introduction to R is available in PDF format.
- A ssh client program (ftp) for windows can be installed on your private computers, allowing you to remote login to the CBS computers.
2-Jan-2006 (Monday): Introduction to microarray technology and R software
- 09.00-09.30
- Introduction to course (Lecture slides)
- H. Bjørn Nielsen
- 09.30-09.45
- Pre-test
- H. Bjørn Nielsen
- 9.45-10.30
- Overview over gene expression and DNA microarray technology (Chapter 1)(Lecture slides)
- Jeppe S. Spicker
- 10.30-10.45
- Break
- 10.45-11.45
- Case story: Analysis of the cell cycle (Lecture slides,de Lichtenberg et al. 2004)
- Ulrik de Lichtenberg
- 11.45-12.15
- Demonstration of microarray equipment at CBS (in groups)
- Tim Binnewies and Thomas Jensen
- 12.15-13.00
- Lunch Break
- 13.00-13.30
- Introduction to UNIX and R (Lecture slides)
- Carsten Friis
- 13.30-15.15
- Exercise: Introduction to UNIX and R (Exercise,Extra exercise)
- Carsten Friis, Jeppe S. Spicker, Agnieszka Juncker and H. Bjørn Nielsen
- 15.15-15.30
- Test of the day
- Jeppe S. Spicker
3-Jan-2006 (Tuesday): Preprocessing and Bioconductor
- 09.00-09.45
- Hybridization, scanning and image analysis (Lecture slides)
- H. Bjørn Nielsen
- 09.45-10.45
- Project introduction - group formation
- H. Bjørn Nielsen
- 10.45-11.00
- Break
- 11.00-12.00
- Case story: Disease Gene Finding, (poster)
- Kasper Lage Hansen
- 12.00-13.00
- Lunch Break
- 13.00-13.30
- Introduction to Bioconductor (Lecture slides)
- Carsten Friis
- 13.30-14.30
- Exercise: Introduction to Bioconductor (Exercise)
- Carsten Friis, Hanni Willenbrock and Jeppe S. Spicker
- 14.30-14.45
- Break
- 14.45-16.15
- Public data - available for project (1)
- Agnieszks Junker
- 16.15-16.30
- Test of the day
- H. Bjørn Nielsen
4-Jan-2006 (Wednesday): Data Normalization
- 09.00-10.00
- Normalization and expression index calculation (Section 3.1-3.4) (Lecture slides)
- H. Bjørn Nielsen
- 10.00-10.15
- Break
- 10.15-12.00
- Exercise: Normalization methods and expression index calculation (Exercise)
- Carsten Friis, Hanni Willenbrock and Jeppe S. Spicker
- 12.00-13.00
- Lunch Break
- 13.00-14.00
- Case story: A key regulator of pathogen defense responses in Arabidopsis (Petersen et al. 2000)
- H. Bjørn Nielsen
- 14.00-14.15
- Break
- 14.15-15:00
- Project work - problem formulation
- 15.00-15.30
- Case story: Epistasis by microarray
- H. Bjørn Nielsen
- 15.30-15.45
- Test of the day
- H. Bjørn Nielsen
5-Jan-2006 (Thursday): Statistical analysis
- 09.00-10.00
- Statistical analysis (Section 3.5-3.6) (Lecture slides)
- H. Bjørn Nielsen
- 10.00-10.15
- Coffee Break
- 10.15-12.00
- Exercise: Statistical analysis including multiple testing correction (Exercise,data.tab, annotation.tab, Lecture)
- Jeppe S. Spicker, Hanni Willenbrock and Carsten Friis
- 12.00-13.00
- Lunch Break
- 13.00-13.45
- Case story: Regulation of the storage protein hordein C production in barley - How to reduce N-waste
- Michael Hansen
- 13.45-14.00
- Break
- 14.00-16.00
- Public data - available for project (Human tissue, Spike-in, Cell cycle)
- Carsten Friis
- 16.00-16.15
- Test of the day
- H. Bjørn Nielsen
6-Jan-2006 (Friday): Data visualization and class prediction
- 09.00-09.45
- Dimension reduction: PCA and Clustering (Chapter 4 and 5) (Lecture slides)
- Agnieszka Juncker
- 09.45-10.00
- Break
- 10.00-11.15
- Exercise: Dimension reduction: PCA and Clustering (Exercise)
- Jeppe S. Spicker and Agnieszka Juncker
- 11.15-12.00
- Classification (Chapter 8) (Lecture slides)
- Agnieszka Juncker
- 12.00-13.00
- Lunch Break
- 13.00-14.00
- Case story: Predicting Toxicity - An Unfinished Case Story
- Jeppe S. Spicker
- 14.00-14.15
- Break
- 14.15-15.00
- Exercise: Classification, (Exercise)
- Jeppe S. Spicker and Agnieszka Juncker
- 15.00-15.15
- Test of the day
- Agnieszka Juncker
9-Jan-2006 (Monday): Gene annotation analysis
- 09.00-11.00
- Gene Onthology (GO), KEGG and ChIp-chip (Lecture slides, Exercise, Solutions)
- Agnieszka Juncker and Hanni Willenbrock
- 11.00-11.15
- Break
- 11.15-12.00
- Case story: F.A.R.O.
- 12.00-13.00
- Lunch Break
- 13.00-13.45
- Case story: DNA Chip prognosis of breast cancer - a meta analysis
- Steen Knudsen
- 13.45-14.00
- Break
- 14.00-16.00
- Public data - available for project (Leukemia, Plant mutants, Rosetta compendium)
- H. Bjørn Nielsen
- 15.15-15.30
- Test of the day
- Agnieszka Juncker
10-Jan-2006 (Tuesday): Integrative Biology
- 09:00-11:00
- Integrative Biology (with exercise)
- Carsten Friis
- 11:00-12:00
- Project work - (project outline)
- 12.00-13.00
- Lunch Break
- 13.00-13.45
- Comparative Genomic Hybridization (CGH)
- Hanni Willenbrock
- 13.45-14.00
- Test of the day
- Hanni Willenbrock
11-Jan-2006 (Wednesday): Project Problem Formulation
- 09.00-11.00
- Probe design using OligoWiz 2.0 (lecture notes, Exercise guide, OligoWiz 2.0 server
- Rasmus Wernersson
- 11.00-11.15
- Break
- 11.15-12.00
- Case story: From genome sequence to gene expression data in Campylobacter jejuni
- Peter F. Hallin
- 12.00-13.00
- Lunch Break
- 13:00-14:00
- Project work - (Deadline for Problem formulation - hand in written P.F.)
- 14.00-14.15
- Break
- 14:15-15:15
- Exercise: Reading Data into R (Exercise)
- Carsten Friis
- 15.15-15.30
- Test of the day
- Rasmus Wernersson
12-Jan-2006 (Thursday): Problem Presentations
- 09.00-10.30
- Promoter analysis (Lecture slides, Exercise)
- H. Bjørn Nielsen and Agnieszka Juncker
- 10.30-10.45
- Break
- 10.45-12.00
- Problem presentations (5min pr. group, one slide)
- Project groups
- 12.00-13.00
- Lunch Break
- 13.00-13.30
- course summary
- H. Bjørn Nielsen
- 13.30-
- Project work
13-Jan-2006 (Friday) — 19-Jan-2006 (Thursday): Project Work
- 10.00-12.00
- Office Hours - one or more Teacher(s) can be found in auditorium 51
20-Jan-2006 (Friday): Poster Session
- 10.00-15.00
- Poster Session (exam) - In the hall infront of CBS (building 208)
18-Jan-2006 (Wednesday) — 21-Jan-2006 (Saturday)
- Course evaluation on CampusNet
- Please, use your student number and password to login