Talk:Perl

Perl and MySQL

This section will just list a bunch of random examples. They are useful to give you an idea of what you can do with Perl, MySQL, and the CLI.

Note: These examples were taken from my course in Comparative Microbial Genomics at CBS (in Denmark).

mysql -B -e "update cmp_genomics.features set note = '' where user = USER() and note not like 'tcs%' or note is null"
foreach accession (AE017042 AE016879 AL111168 AL645882 AP008232 AP009048 BA000021 CP000034)
   foreach type (ecf s54 s70)
     cat source/$accession.proteins.$type.sigmas.hmmsearch | \
     perl -ne 'next unless /^CDS_(\d+)\-(\d+)_DIR([\-\+]+)\s+([0-9\-\.e]+)\s+([0-9\-\.e]+)\s+/;\
               my ($start,$stop,$dir,$score,$evalue) = ($1,$2,$3,$4,$5);\
               next unless $score > 0;\
               print "update cmp_genomics.features set note = \"Sigma Factor '$type'\" \
                      where start=$start and stop = $stop and user = user() and accession = \"'$accession'\";\n";'\
     | mysql
   end
end

mysql -B -e "update cmp_genomics.features set note = '' where user = USER() and note not like 'sigma%' or note is null"
foreach accession (AE017042 AE016879 AL111168 AL645882 AP008232 AP009048 BA000021 CP000034)
   foreach type (RRreciever HisKA_1 HisKA_2 HisKA_3 HWE_HK)
     cat source/$accession.$type.TCS.hmmsearch | \
     perl -ne 'next unless /^CDS_(\d+)\-(\d+)_DIR([\-\+]+)\s+([0-9\-\.e]+)\s+([0-9\-\.e]+)\s+/;\
               my ($start,$stop,$dir,$score,$evalue) = ($1,$2,$3,$4,$5);\
               next unless $score > 0; \
               print "update cmp_genomics.features set note = \"TCS '$type'\" \
                      where start=$start and stop = $stop and user = user() and accession = \"'$accession'\";\n";'\
     | mysql -B
   end
end

rm -f source/all.16s.fsa
foreach accession (AE017042 AE016879 AL111168 AL645882 AP008232 AP009048 BA000021 CP000034)
   mysql -B -N -D cmp_genomics -e "select substr(seq,start,stop-start+1) as sequence,\
     replace(substring_index(genomes.organism,' ',4),' ','_') as name,dir from genomes,features where \
     genomes.accession = features.accession and genomes.user = features.user and \
     features.user = user() and featuretype='rRNA' and features.accession = '$accession'" | \
   perl -ane '$F[0] = reverse ( $F[0] ) if $F[2] eq "-";$F[0] =~ tr/ATGC/TACG/ if $F[2] eq "-";\
              print ">$F[1]\n"; \
              for ( $x = 0 ; $x < length ( $F[0] ) ; $x = $x+60) { print substr ( $F[0] , $x , 60),"\n"; } last;' \
   >> source/all.16s.fsa
end
less source/all.16s.fsa

cat ~/Ex3/source/CP000034.codon-usage.aa.list \
| perl -ane 'next unless $F[1] =~ /^[A-Z]+/;print "\t$F[1]"; ' \
> aa.dat
mysql -B -N -e 'select accession,organism from cmp_genomics.genomes where user=USER()' \
> organism.names
foreach accession (AE017042 AE016879 AL111168 AL645882 AP008232 AP009048 BA000021 CP000034)
  cat ~/Ex3/source/$accession.codon-usage.aa.list \
  | perl -ane 'BEGIN { foreach (`cat organism.names`) \
                       {chomp; @A = split (/\t+/,$_);$NAMES{$A[0]} = $A[1];} } \
               next unless $F[1] =~ /^[A-Z]+/;$FREQ{$F[1]} = $F[3]; push @AA,$F[1]; \
               END { print "\n"; print "$NAMES{'$accession'}"; foreach $aa (@AA){print "\t",$FREQ{$aa};}}' \
  >> aa.dat
end

mysql -e "delete from localization where user=USER() and type='signalp'" cmp_genomics
foreach accession (AE017042 AE016879 AL111168 AL645882 AP008232 AP009048 BA000021 CP000034)
  perl -ne 'if(/^CDS_(\d+)-(\d+)_DIR([+-])\s+0.\d+\s+\d+\s+[YN]\s+0.\d+\s+\d+\s+[YN]\s+[10].\d+\s+\d+\s+Y\s+[10].\d+\s+Y\s+[10].\s+\s+Y\s+CDS_\d+-\d+_DIR[+-]\s+Q\s+[10].\d+\s+[10]\s+Y\s+[10].\d+\s+Y/) \
            {print "insert into localization (user, protid, type) \
                           select USER() as user, ft.id as protid, \"signalp\" as type \
                                  from features as ft \
                                  where ft.user=USER() and \
                                        ft.accession = \"'$accession'\" and \
                                        ft.start = $1 and ft.stop = $2 and \
                                        ft.dir = \"$3\";\n";}' $accession.signalp \
  | mysql -D cmp_genomics
end

perl -e 'foreach $i (1..11) { open IN , "col$i"; while (<IN>) { chomp;($a , $c) = split (/\t+/,$_); $DATA{$a}{$i} = $c;} close IN;} \
         END {foreach $a ( reverse sort keys %DATA) {foreach $i (1..11) {$DATA{$a}{$i} = 0 if $DATA{$a}{$i} eq "" and $i > 1; \
         print $DATA{$a}{$i}; print "\t" if $i < 11;}print "\n";}}' \
> final.dat

R
postscript ('final.ps')
source ( "/home/people/kiil/Exercises/files/heatmap.R" )
data <- as.matrix ( read.table ( 'final.dat' , row.names=1, header= TRUE,sep = "\t" ) )
pal <- maPalette(low = rgb(10,10,10,maxColorValue = 10), high = rgb(0,0,10,maxColorValue = 10), mid= rgb(5,5,10,maxColorValue = 10),, k = 1024)
par(oma=c(0, 0, 0, 0.5))
data  <- scale(data)
new_heatmap ( data , cexCol=1,cexRow=1,colors = pal)
dev.off()
quit(save="no")