Biochemistry (academic course)
Biochemistry was a year-long academic course I took during my undergraduate years at Oregon State University. It was a sequence professional course to meet the requirements of majors in Biochemistry and Biophysics.
- Professors: Dr. Michael I. Schimerlik, Dr. Tory M. Hagen, Dr. Christopher K. Mathews, and Dr. George D. Pearson.
- Textbook: Biochemistry, Christopher K. Mathews, K. E. van Holde, and Kevin G. Ahern, Addison Wesley Longman, San Fransisco, 3rd Edition (2000).
The course was divided into three terms and there was also separate courses for the laboratory.
Energetics of Life (Thermodynamics, Chemical Reactions and Equilibrium), Nucleic Acids (Properties, Structure, Function), Protein Structure, Protein Function and Evolution, Protein Dynamics, Carbohydrates.
Keywords: Chemistry of amino acids and proteins; specialized proteins, including heme proteins and muscle proteins; enzymes, including kinetics and mechanisms; bioenergetics, thermodynamics, and biological oxidation and reduction; carbohydrate chemistry; introduction to metabolism; glycolysis; glycogen metabolism; gluconeogenesis.
Lipids, Membranes, Cellular Transport, Enzymes, Carbohydrate Metabolism, Oxidative Processes, Photosynthesis, Lipid Metabolism, Metabolism of Nitrogenous Compounds, Nucleotide Metabolism, Metabolic Coordination/Control/Signal Transduction.
Keywords: Bioenergetics; lipids and membranes; energy-yielding metabolic pathways, oxidative phosphorylation, and photosynthesis; chemistry and metabolism of lipids, steroids, and eicosanoids; membrane structure, hormone action; metabolic regulation; amino acid and nitrogen metabolism; nucleotide metabolism.
Information Copying (Replication), Restriction, Repair, Recombination, Rearrangement, Amplification, Transcription, Translation, Expression.
Keywords: Nucleic acid structure; replication, transcription, translation; regulation of gene expression; DNA repair, biochemistry of specialized cell functions; oncogenes and growth control.
Fundamentals of biochemical methodology: buffers, spectrophotometry, gel electrophoresis, thin-layer chromatography, protein purification, enzyme kinetics, peptide structure, preparative ultracentrifugation, scientific report writing.
Textbook: Fundamental Laboratory Approaches for Biochemistry and Biotechnology, Ninfa and Ballou.
Research techniques in molecular genetics and nucleic acid biochemistry: Plasmid isolation, restriction analysis, molecular cloning, PCR, site-directed mutagenesis, overexpression of proteins in E. coli, purification of recombinant proteins, analysis of proteins by SDS-PAGE and western blotting, computer-aided DNA sequence analysis, nucleic acid hybridization.
Microdetection, quantitation and communication. Focus upon radioisotopes.