Dr. Alex Rich Laboratory
The Dr. Alex Rich Laboratory is where I did scientific research during Summer 2002/December 2002. It was located in the Department of Biology, Massachusetts Institute of Technology (MIT), USA, Summer 2002/December 2002.
In 1979, Rich and co-workers at MIT accidentally grew a crystal of Z-DNA. Because of my research at OSU on Z-DNA and its biological role and because Dr. Rich is one of the leading experts on Z-DNA, I went to work for him at MIT.
In order to further study the biological role of Z-DNA in the regulation of transcription in various organisms, an experiment was designed to test just this. I ran a microarray of all open reading frames (ORFs) in yeast and measured the role of those ORFs having Zα-binding protein in solution (which should bind near their transcriptional start sites, if our hypothesis is correct) against the wild type. These were run on Affymetrix GeneChips and analyzed with proprietary software from Affymetrix.
Our collaboration resulted in a paper published on Z-DNA and a possible biological role:
- P. Christoph Champ, Sandor Maurice, Jeffery M. Vargason, Tracy Camp, and P. Shing Ho (2004). "Distributions of Z-DNA and nuclear factor I in human chromosome 22: a model for coupled transcriptional regulation". Nucleic Acids Research, 32(22):6501-6510.